Effects of continuous haemofiltration on serum enzyme concentrations, endotoxemia, homeostasis and survival in dogs with severe heat stroke



      To examine the effectiveness of continuous haemofiltration as a treatment for severe heat stroke in dogs.


      Dogs were randomly allocated to a control or continuous haemofiltration group (both n = 8). Heat stroke was induced by placing anaesthetised dogs in a high temperature cabin simulator. Upon confirmation of heat stroke (rectal temperature > 42 °C, mean arterial pressure (MAP) decrease > 25 mmHg), dogs were removed from the chamber and continuous haemofiltration was initiated and continued for 3 h for dogs in the continuous haemofiltration group. Dogs in the control group were observed at room temperature.


      Rectal temperature, haemodynamics, pH, blood gases and electrolyte concentrations rapidly returned to baseline in the continuous haemofiltration group, but not the control group. After 3 h, rectal temperature was 36.68 ± 0.51 °C in the continuous haemofiltration group and 39.83 ± 1.10 °C in the control group (P < 0.05). Continuous haemofiltration prevented endotoxin and all serum enzyme concentrations from increasing and caused malondialdehyde concentrations to decrease. After 3 h, endotoxin concentrations were 0.14 ± 0.02 EU ml−1 in the continuous haemofiltration group and 0.23 ± 0.05 EU ml−1 in the control group (P = 0.003), while malondialdehyde concentrations were 4.86 ± 0.61 mmol l−1 in the continuous haemofiltration group and 8.63 ± 0.66 mmol l−1 in the control group (P < 0.001). Five dogs died in the control group within 3 h, whereas no dogs died in the continuous haemofiltration group.


      Continuous haemofiltration rapidly reduced body temperature, normalised haemodynamics and electrolytes, improved serum enzyme concentrations and increased survival in dogs with heat stroke. Continuous haemofiltration may be an effective treatment for heat stroke.


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        • Leon L.R.
        • Helwig B.G.
        Heat stroke: role of the systemic inflammatory response.
        J Appl Physiol. 2010; 109: 1980-1988
        • Wallace R.F.
        • Kriebel D.
        • Punnett L.
        • Wegman D.H.
        • Amoroso P.J.
        Prior heat illness hospitalization and risk of early death.
        Environ Res. 2007; 104: 290-295
        • Leon L.R.
        • Helwig B.G.
        Role of endotoxin and cytokines in the systemic inflammatory response to heat injury.
        Front Biosci (Schol Ed). 2010; 2: 916-938
        • Argaud L.
        • Ferry T.
        • Le Q.H.
        • et al.
        Short- and long-term outcomes of heatstroke following the 2003 heat wave in Lyon, France.
        Arch Intern Med. 2007; 167: 2177-2183
        • Dematte J.E.
        • O’Mara K.
        • Buescher J.
        • et al.
        Near-fatal heat stroke during the 1995 heat wave in Chicago.
        Ann Intern Med. 1998; 129: 173-181
        • Saito A.
        Current progress in blood purification methods used in critical care medicine.
        Contrib Nephrol. 2010; 166: 100-111
        • Wakino S.
        • Hori S.
        • Mimura T.
        • et al.
        Heat stroke with multiple organ failure treated with cold hemodialysis and cold continuous hemodiafiltration: a case report.
        Ther Apher Dial. 2005; 9: 423-428
        • Yue H.
        • Zhou F.
        • Liu H.
        • et al.
        Fatal exertional heat stroke successfully treated with cold hemofiltration: a case report.
        Am J Emerg Med. 2009; 27: 751e1-751e2
        • Ikeda Y.
        • Sakemi T.
        • Nishihara G.
        • et al.
        Efficacy of blood purification therapy for heat stroke presenting rapid progress of multiple organ dysfunction syndrome: a comparison of five cases.
        Intensive Care Med. 1999; 25: 315-318
        • Amsterdam J.T.
        • Syverud S.A.
        • Barker W.J.
        • et al.
        Dantrolene sodium for treatment of heatstroke victims: lack of efficacy in a canine model.
        Am J Emerg Med. 1986; 4: 399-405
        • Yan Y.E.
        • Zhao Y.Q.
        • Wang H.
        • Fan M.
        Pathophysiological factors underlying heatstroke.
        Med Hypotheses. 2006; 67: 609-617
        • Lucendo Villarín A.J.
        • Carrión Alonso G.
        • Kassem A.
        • Pajares Villarroya R.
        • Manceñido Marcos N.
        • Rey Sanz R.
        Bruising of the esophagus as a cause of gastrointestinal bleeding in a case of heatstroke.
        Rev Esp Enferm Dig. 2005; 97: 596-599
        • Yao X.
        • Feng Z.T.
        • Liu W.
        Comparisons of temperature lowering measures through colon and with ice bag in heat shock dogs.
        Chin J Mult Organ Dis Elder. 2007; 6: 349
        • Alzeer A.H.
        • el-Hazmi M.A.
        • Warsy A.S.
        • Ansari Z.A.
        • Yrkendi M.S.
        Serum enzymes in heat stroke: prognostic implication.
        Clin Chem. 1997; 43: 1182-1187
        • Naka T.
        • Haase M.
        • Bellomo R.
        ‘Super high-flux’ or ‘high cut-off’ hemofiltration and hemodialysis.
        Contrib Nephrol. 2010; 166: 181-189
        • Li L.
        • Pan J.
        • Yu Y.
        Development of sorbent therapy for multiple organ dysfunction syndrome (MODS).
        Biomed Mater. 2007; 2: R12-R16